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61.
Contribution of solvent drag through intercellular junctions to absorption of nutrients by the small intestine of the rat 总被引:19,自引:0,他引:19
Summary The lumen of the small intestine in anesthetized rats was recirculated with 50 ml perfusion fluid containing normal salts, 25mm glucose and low concentrations of hydrophilic solutes ranging in size from creatinine (mol wt 113) to Inulin (mol wt 5500). Ferrocyanide, a nontoxic, quadrupally charged anion was not absorbed; it could therefore be used as an osmotically active solute with reflection coefficient of 1.0 to adjust rates of fluid absorption,J
v
, and to measure the coefficient of osmotic flow,L
p
. The clearances from the perfusion fluid of all other test solutes were approximately proportional toJ
v
. FromL
p
and rates of clearances as a function ofJ
v
and molecular size we estimate (a) the fraction of fluid absorption which passes paracellularly (approx. 50%), (b) coefficients of solvent drag of various solutes within intercellular junctions, (c) the equivalent pore radius of intercellular junctions (50 Å) and their cross sectional area per unit path length (4.3 cm per cm length of intestine). Glucose absorption also varied as a function ofJ
v
. From this relationship and the clearances of inert markers we calculate the rate of active transport of glucose, the amount of glucose carried paracellularly by solvent drag or back-diffusion at any givenJ
v
and luminal glucose concentration and the concentration of glucose in the absorbate. The results indicate that solvent drag through paracellular channels is the principal route for intestinal transport of glucose or amino acids at physiological rates of fluid absorption and concentration. In the absence of luminal glucose the rate of fluid absorption and the clearances of all inert hydrophilic solutes were greatly reduced. It is proposed that Na-coupled transport of organic solutes from lumen to intercellular spaces provides the principal osmotic force for fluid absorption and triggers widening of intercellular junctions, thus promoting bulk absorption of nutrients by solvent drag. Further evidence for regulation of channel width is provided in accompanying papers on changes in electrical impedance and ultrastructure of junctions during Na-coupled solute transport. 相似文献
62.
Preliminary data on the skeletal biology of 78 Taino skeletons belonging to Juan Dolio, an archaeological site of the Maguana
province, 80 Km. east of S. Domingo, are presented. The minimum number of individuals, sex, age, stature, and morphologic
and morphometric characters were determined. Dental wear and pathology of cranial and post-cranial bones were also recorded. 相似文献
63.
Takakazu Yumoto 《Ecological Research》1987,2(2):133-145
Animal pollination in a warm temperate evergreen broad-leaved forest was observed on Yaku-shima Island, south of Kyushu, Japan.
Three groups of plants were categorized: canopy-flowering tree species, understory-flowering tree species, and climber and
epiphyte species. Each of these formed different pollination systems. The canopy-flowering tree species had shallow, dish-shaped
flowers and utilized various types of opportunistic pollinators. Most of the climber and epiphyte species had deep, tube-shaped
flowers and specialized pollinators, although some climber species which bloomed in the canopy especially in winter, had opportunistic
pollinators. The understory-flowering tree species had large dish- or funnel-shaped flowers and endothermic pollinators able
to tolerate the dark and cold conditions under the canopy. The individual trees of canopy-flowering tree species produced
large numbers of flowers simultaneously (mass-flowering) and had a well synchronized flowering period. Each canopy-flowering
tree species segregated its flowering time from those of the anothers. Climber and epiphyte species and most of the understory-flowering
tree species produced small numbers of flowers sequentially (extended flowering) and showed a long flowering period. 相似文献
64.
The low molecular weight carbohydrate compositions of the seeds of 29 species ofVicia, namelyV. amoena, V. amurensis, V. bifolia, V. dumetorum, V. fauriei, V. japonica, V. nipponica, V. pisiformis, V. pseudo-orobus,
V. sylvatica, V. unijuga, V. venosa, V. cassubica, V. orobus, V. cracca agg.,V. hirsuta, V. villosa agg.,V. tetrasperma,V. oroboides, V. sepium, V. cuspidata, V. grandiflora, V. lathyloides, V. sativa agg.,V. bithynica, V. faba, V. narbonensis, V. hybrida andV. lutea were determined by gas liquid chromatography. The carbohydrate compositions were found to be species-specific. Principal
component analysis of the carbohydrate composition data showed that these species can be divided into three groups. Although,
as far as the examined species were concerned, these groups were not correlated with the known subgenera, significant correlation
between the groups and the known sections was detected in the subgenusVicia. The carbohydrate composition character would be important to clarify the relationships among closely related taxa of the
genusVicia. 相似文献
65.
J. B. T. M. Roerdink 《Journal of mathematical biology》1989,27(3):309-319
In a previous paper (J. Math. Biol. 26, 199–215 (1988)) we calculated the mean and variance of the long-run geometric growth rate of a discrete-time population model with two age classes in a random environment. The formula which was used in that paper as the starting point for the computation of the variance represents only the contribution of the one-period variances. Here we supplement these results by a calculation of the exact variance. All qualitative conclusions reached before are unaffected. 相似文献
66.
1. The working hypothesis that neuropeptide gene expression in a neuron is an indicator of that neuron's physiological activity is discussed. 2. Representative examples from the literature are presented to support the hypothesis. 3. Further, we discuss the regulation of expression of two opioid peptides, preproenkephalin and preprodynorphin, in laminae I and II of the spinal cord and in nucleus caudalis of the trigeminal nuclear complex, where they may play a role in pain modulation. 4. The expression of the opioid peptide genes can be induced by both painful and nonnoxious stimuli in neurons in time-dependent and sensory-specific fashions. 相似文献
67.
S Swaminathan G Ravishanker D L Beveridge R Lavery C Etchebest H Sklenar 《Proteins》1990,8(2):179-193
A new procedure for the graphic analysis of molecular dynamics (MD) simulations on proteins is introduced, in which comprehensive visualization of results and pattern recognition is greatly facilitated. The method involves determining the conformational and helicoidal parameters for each structure entering the analysis via the method "Curves," developed for proteins by Sklenar, Etchebest, and Lavery (Proteins: Structure, Function Genet. 6:46-60, 1989) followed by a novel computer graphic display of the results. The graphic display is organized systematically using conformation wheels ("dials") for each torsional parameter and "windows" on the range values assumed by the linear and angular helicoidal parameters, and is present in a form isomorphous with the primary structure per se. The complete time evolution of dynamic structure can then be depicted in a set of four composite figures. Dynamic aspects of secondary and tertiary structure are also provided. The procedure is illustrated with an analysis of a 50 psec in vacuo simulation on the 58 residue protein, bovine pancreatic trypsin inhibitor (BPTI), in the vicinity of the local minimum on the energy surface corresponding to a high resolution crystal structure. The time evolution of 272 conformational and 788 helicoidal parameters for BPTI is analyzed. A number of interesting features can be discerned in the analysis, including the dynamic range of conformational and helicoidal motions, the dynamic extent of 2 degrees structure motifs, and the calculated fluctuations in the helix axis. This approach is expected to be useful for a critical analysis of the effects of various assumptions about force field parameters, truncation of potentials, solvation, and electrostatic effects, and can thus contribute to the development of more reliable simulation protocols for proteins. Extensions of the analysis to present differential changes in conformational and helicoidal parameters is expected to be valuable in MD studies of protein complexes with substrates, inhibitors, and effectors and in determining the nature of structural changes in protein-protein interactions. 相似文献
68.
Two regions of the ribosomal DNA (rDNA) were sequenced from a range of species from the tribeTriticeae. One region, the central spacer, was found to be more divergent in sequence than the other, the 18 S-spacer junction. Both regions contained sequences 20–30 bp long which were more highly conserved than the remainder of the region and their possible significance in rDNA expression is discussed. Phenetic relationships based on the sequence data were generally consistent with the relationships based on other criteria. Species possessing the S, E, J1J2, D, and B genomes clustered together, with the H genome species being the most distinct of those examined. The R, P, and V genome species occupy an intermediate position in the overall pattern of relationships. Some relationships differed in detail from those established by other parameters, for example the position of the N genome species, and explanations for discrepancies of this type are discussed. 相似文献
69.
Data on the physico-chemical properties of proteins from soybean, groundnut, sesame seed, sunflower seed, safflower seed,
mustard seed, rapeseed and cotton seed are fairly extensive. An examination of the available data on high molecular weight
proteins suggests that there are similarities in many of their properties. In this report the similarity in amino acid composition,
size and shape, molecular weight, secondary structure, subunit composition, association-dissociation at high and low pH, stability
towards denaturants, hydrolysis by enzymes and quaternary structure of the high molecular weight proteins is discussed. Based
on these similarities a model has been proposed for the associationdissociation, denaturation and reassociation behaviour
of the high molecular weight proteins of oilseeds. 相似文献
70.
Summary Little is known of the response of mollusc populations to predation by humans, particularly for tropical species. In this paper, we examine the effects of human predation on populations of the gastropod Strombus luhuanus in Bootless Inlet, Papua New Guinea, by documenting both the population biology of the shellfish and the shell-gathering practices of traditional and contemporary human groups. Strombus luhuanus occurs in local colonies and individuals of each sex from different colonies differed significantly in size. Sexual maturity is reached within two years after settlement, at which time the shell length stabilises at about 35–60 mm, and the shell lip thickens. There was also significant between-colony variation in density (8.35–23.39 individuals/m2), and colonies differed in the depth range of their distributions and the frequency of human collection visits. Traditional gatherers rarely collected individuals which were buried or subtidal. Contemporary collectors used different collecting methods, and gathered subtidal populations to a depth of 2.5 m. Both traditional and contemporary collectors gathered only individuals greater than 30 mm shell length, and in the contemporary sample the probability of being gathered increased significantly with shell length. This was due to size-dependent burying, which was greatest among young juveniles and least among adults. The traditional sample contained fewer shells in the largest size category (>45 mm) and more in the smallest (<40 mm), but this difference largely represents the pooling of shells from different collecting locations rather than widespread juvenisation of colonies due to exploitation. Stromb population densities at collected sites in PNG far exceeded those in comparable uncollected sites in northeastern Australia. We conclude that S. luhuanus displays high resilience to all gathering practices used to date, as a consequence of both its size-dependent burying and partly subtidal distribution, which provide refugia from human predation. 相似文献